Mapping molecules in scanning far-field fluorescence nanoscopy
نویسندگان
چکیده
منابع مشابه
Mapping molecules in scanning far-field fluorescence nanoscopy
In fluorescence microscopy, the distribution of the emitting molecule number in space is usually obtained by dividing the measured fluorescence by that of a single emitter. However, the brightness of individual emitters may vary strongly in the sample or be inaccessible. Moreover, with increasing (super-) resolution, fewer molecules are found per pixel, making this approach unreliable. Here we ...
متن کاملTwo-color far-field fluorescence nanoscopy.
We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and red-emitting fluorophores are selectively excited and quenched using dedicated beam pairs. The stimulated emission depletion beams deliver a lateral resolution of <30 nm and 65 nm for the green...
متن کاملFar - Field Optical Nanoscopy
37. E. Evans, Annu. Rev. Biophys. Biomol. Struct. 30, 105 (2001). 38. G. I. Bell, Science 200, 618 (1978). 39. For adhesive interactions important in soft tissues and organs of eukaryotic cell systems, the average times toff° reported for spontaneous dissociation of ligand/receptor bonds range from a fraction of a second to 100 s or more. 40. Because of thermal activation, the appropriate unit ...
متن کاملFar-field autofluorescence nanoscopy.
We demonstrate far-field optical imaging at the nanoscale with unlabeled samples. Subdiffraction resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning. Our approach is based on the insight that nanoscopy methods...
متن کاملFar - Field Optical Nanoscopy Stefan
37. E. Evans, Annu. Rev. Biophys. Biomol. Struct. 30, 105 (2001). 38. G. I. Bell, Science 200, 618 (1978). 39. For adhesive interactions important in soft tissues and organs of eukaryotic cell systems, the average times toff° reported for spontaneous dissociation of ligand/receptor bonds range from a fraction of a second to 100 s or more. 40. Because of thermal activation, the appropriate unit ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Communications
سال: 2015
ISSN: 2041-1723
DOI: 10.1038/ncomms8977